RNase-Free DNase Set 試劑盒耗材 OMEGA

描述：RNase-Free DNase Set
用于RNA提取時(shí)DNA的膜上消化以去除DNA污染

E.Z.N.A.RNA抽提試劑盒可以去除大量的基因組DNA污染,對(duì)大多數(shù)的下游實(shí)驗(yàn),如Northern雜交并不需要特別的處理

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更新時(shí)間：2015-11-16

產(chǎn)品型號(hào)：E1091-01

所在城市：上海市

廠商性質(zhì)：經(jīng)銷商

訪問量：6130

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RNase-Free DNase Set

用于RNA提取時(shí)DNA的膜上消化以去除DNA污染

E.Z.N.A.RNA抽提試劑盒可以去除大量的基因組DNA污染,對(duì)大多數(shù)的下游實(shí)驗(yàn),如Northern雜交并不需要特別的處理,但是對(duì)于RT-PCR而言,即便是幾個(gè)分子的核酸片段都可能被檢測(cè)到.因此RNA中微量的基因組DNA污染對(duì)這種靈敏的實(shí)驗(yàn)有著非常大的干擾.目前還沒有一個(gè)抽提試劑盒可*去除DNA的污染,除非使用DNase進(jìn)行處理. 該試劑盒采用無RNase酶的DNase,專門為膜上DNA酶消化而設(shè)計(jì)的.在使用E.Z.N.A.RNA Kit抽提RNA時(shí),當(dāng)RNA結(jié)合到硅膠柱的膜上以后,可以在柱子中加入DNase和特殊的消化緩沖液,孵育酶切DNA,然后再加入洗滌液洗滌去除降解的DNA,DNase和鹽,后洗脫出RNA.使用該方法得到的RNA可確保不存在DNA的污染和DNase的污染.

產(chǎn)品特性與優(yōu)點(diǎn)

定購(gòu)信息

編碼	名稱	次數(shù)	詳情	單價(jià)
E1091-01	RNase-Free DNase Set	50	1500 Units	500.00
E1091-02	RNase-Free DNase Set	200	6000 Units	1800.00

顯示詳細(xì)信息

PCR與RT-PCR相關(guān)
Taq DNA Polymerase:DNA聚合酶
TQ2100-00	Taq DNA Polymerase(500U)	160
TQ2100-01	Taq DNA Polymerase(1000U)	300
TQ2100-02	Taq DNA Polymerase(5000U)	1300
TQ2100-03	Taq DNA Polymerase(10000U)	2200
PCR Amplification Kit
TQ2104-01	PCR Amplification Kit(500U)	250
TQ2104-02	PCR Amplification Kit(1000U)	480
TQ2104-02	PCR Amplification Kit(5000U)	2300
Pfu DNA polymerase:pfu DNA聚合酶
TQ2101-00	Pfu DNA Polymerase(250U)	180
TQ2101-01	Pfu DNA Polymerase(500U)	350
Perfectstart Taq DNA polymerase:熱啟動(dòng)Taq DNA聚合酶
TQ2102-00	Perfectstart Taq DNA polymerase(500U)	800
TQ2102-01	Perfectstart Taq DNA polymerase(1000U)	1500
Taq plus DNA polymerase:Taq plus DNA聚合酶
TQ2103-00	Taq Plus DNA Polymerase(500U)	250
TQ2103-01	Taq Plus DNA Polymerase(1000U)	480
Taq master mix:Taq PCR預(yù)混液
TQ2201-01	2 X Taq MasterMixture(5x1ml)含染料	500
TQ2201-02	2 X Taq MasterMixture(20x1ml)含染料	1800
TQ2201-03	2 X Taq MasterMixture(50x1ml)含染料	4250
TQ2200-01	2 X Taq MasterMixture(5x1ml)不含染料	450
TQ2200-02	2 X Taq MasterMixture(20x1ml)不含染料	1600
TQ2200-03	2 X Taq MasterMixture(50x1ml)不含染料	4000
Perfectstart Taq master mix:熱啟動(dòng)Taq PCR預(yù)混液
TQ2202-01	Perfectstart Taq master 2X Mix(5x1ml)	1450
TQ2202-02	Perfectstart Taq master 2X Mix(10x1ml)	2600
High-Fidelity PCR master mix:高保真PCR預(yù)混液
TQ2203-01	High-Fidelity PCR master 2X Mix(5x1ml)	700
TQ2203-02	High-Fidelity PCR master 2X Mix(20x1ml)	1200
Perfectstart SYBY Green qPCR master mix:熱啟動(dòng)SYBR Green 實(shí)時(shí)定量PCR預(yù)混液
TQ2300-01	Perfectstart SYBR Green qPCR master mix	600
TQ2300-02	Perfectstart SYBR Green qPCR master mix	2100
Perfectstart Taqman qPCR master mix:熱啟動(dòng)Taqman實(shí)時(shí)定量PCR預(yù)混液
TQ2301-01	Perfectstart Taqman qPCR master mix	600
TQ2301-02	Perfectstart Taqman qPCR master mix	2100
M-MLV transcriptase:M-MLV逆轉(zhuǎn)錄酶
TQ2401-01	M-MLV trancriptase(5000U)	380
TQ2401-02	M-MLV trancriptase(50000U)	3500
M-MLV first strand cDNA Synthesis Kit:M-MLV*鏈cDNA合成試劑盒
TQ2501-01	M-MLV first strand cDNA Synthesis Kit(20)	480
TQ2501-02	M-MLV first strand cDNA Synthesis Kit(100)	2200
One Step RT-PCR kit:一步法反轉(zhuǎn)錄PCR試劑盒
TQ2601-01	Omega One step RT-PCR kit(25)	700
TQ2601-02	Omega One step RT-PCR kit(100)	2700
10mM dNTPs
TQAC135	dNTP solution,10mM(4x250ul)	170
TQAC136	dNTP solution,25mM(4x250ul)	300
100mM dNTP each
TQAC131	dATP solution,100mM(250ul)	200
TQAC132	dcTP solution,100mM(250ul)	200
TQAC133	dGTP solution,100mM(250ul)	200
TQAC134	dTTP solution,100mM(250ul)	200
動(dòng)物組織直接PCR試劑盒：動(dòng)物組織無需經(jīng)DNA抽提而直接用于PCR擴(kuò)增
TQ2600-01	Animal tissue Direct PCR Kit(20) 原名為Tissue Direct PCR Kit(20)	250
TQ2600-02	Animal tissue Direct PCR Kit(100)	1100
TQ2600-03	Animal tissue Direct PCR Kit(500)	5000
TQ2610-01	Fix Direct PCR Kit(20)	350
TQ2610-02	Fix Direct PCR Kit(100)	1500
TQ2610-03	Fix Direct PCR Kit(500)	7000
石蠟組織(FFPE)直接PCR試劑盒：石蠟組織無需經(jīng)DNA抽提而直接用于PCR擴(kuò)增
TQ3000-01	FFPE Direct PCR Kit(20)	300
TQ3000-02	FFPE Direct PCR Kit(100)	1300
TQ3000-03	FFPE Direct PCR Kit(500)	6000
TQ3100-01	microorganism Direct PCR Kit(20)	240
TQ3100-02	microorganism Direct PCR Kit(100)	1100
血液直接PCR試劑盒：血液無需經(jīng)DNA抽提而直接用于PCR擴(kuò)增
TQ2700-01	Blood Direct PCR Kit(20)	250
TQ2700-02	Blood Direct PCR Kit(100)	1100
TQ2700-03	Blood Direct PCR Kit(500)	5000
植物組織直接PCR試劑盒：植物組織無需經(jīng)DNA抽提而直接用于PCR擴(kuò)增
TQ2800-01	Plant tissue Direct PCR kit(20)	220
TQ2800-02	Plant tissue Direct PCR kit(100)	980
TQ2800-03	Plant tissue Direct PCR kit(500)	4500
植物組織直接PCR試劑盒：植物組織無需經(jīng)DNA抽提而直接用于PCR擴(kuò)增
TQ2801-01	Plant tissue Direct PCR kit without Taq(20)	150
TQ2801-02	Plant tissue Direct PCR kit without Taq(100)	650
TQ2801-03	Plant tissue Direct PCR kit without Taq(500)	3000
植物種子直接PCR試劑盒：植物種子無需經(jīng)DNA抽提而直接用于PCR擴(kuò)增
TQ2900-01	Plant seeds Direct PCR kit(20)	250
TQ2900-02	Plant seeds Direct PCR kit(100)	1000
TQ2900-03	Plant seeds Direct PCR kit(500)	5000
50bp DNA Ladder
M11-01	50bp DNA Ladder(250ul)	150
M11-02	50bp DNA Ladder(1ml)	560
50bp DNA Ladder plus
M07-01	50bp DNA Ladder Plus(250ul)	180
M07-02	50bp DNA Ladder Plus (1ml)	680
100bp DNA Ladder
M01-01	100bp DNA Ladder(50)(即250ul)	150
M01-02	100bp DNA Ladder(200)(即1ml)	560
100bp DNA Ladder plus
M08-01	100bp DNA Ladder Plus(250ul)	180
M08-02	100bp DNA Ladder Plus(1ml)	680
200bp DNA Ladder
M02-01	200bp DNA ladder(50)(即250ul)	150
M02-02	200bp DNA Ladder(200)(即1ml)	560
1kb DNA Ladder
M03-01	1kbp DNA Ladder(250ul)(即50次)	90
M03-02	1kbp DNA Ladder(1ml)(即200次)	340
1kb DNA Ladder plus
M09-01	1Kb DNA Ladder Plus(250ul)	160
M09-02	1Kb DNA Ladder Plus(1ml)	600
DL2000 DNA Marker
M10-01	DL2000 DNA Marker(250ul)	85
M10-02	DL2000 DNA Marker(1ml)	320
CL5000 DNA Marker
M06-01	CL5000 DNA Marker (250ul)	150
M06-02	CL5000 DNA Marker (1ml)	560
HT DNA Marker I
M04-01	HT Ladder I(50)(即250ul)	80
M04-02	HT Ladder I(200)(即1ml)	300
HT DNA Marker II
M05-01	HT Ladder II (50)(即250ul)	80
M05-02	HT Ladder II (200)(即1ml)	300

TQR01-01	RNase inhibitor(2000U)	300
TQR01-02	RNase inhibitor(10000U)	1350
耗材與配件、溶液
96孔真空抽濾盒
VAC-03	EZ 96 Vacuum Manifolds	2500
柱式真空抽濾盒
VAC-08	Vacuum Manifolds	1700
磁珠分離架
MSD-01	E-Z 96 Magnetic Separation Device-A	1600
MSD-02	E-Z 96 Magnetic Separation Device-B	1600
MSD-03	Magnetic Separation Device-B	760
2ml收集板
EZ9602-01	96 well 2ml collection plate(5)	250
EZ9602-02	96 well 2ml collection plate(25)	1100
500ul收集板
EZ9604-01	96 well 500ul collection plate(5)	200
EZ9604-02	96 well 500ul collection plate(25)	900
1.2ml帶蓋固定圓底收集板
SSI-1780-01	1.2ml Round Well Plate(10)	500
SSI-1780-02	1.2ml Round Well Plate(100)	3790
1.2ml帶蓋可拆圓底收集板
SSI-1702-00	1.2ml 8-Strip Caps,Non-strerile,125 strips	1500
SSI-1702-S0	1.2ml 8-Strip Caps,Prestrerile,125 strips	1770
SSI-1703-00	1.2ml 8-Strip Caps,Non-strerile,80 strips	1530
SSI-1703-S0	1.2ml 8-Strip Caps,Prestrerile,805 strips	1770
SSI-1760-00	1.2ml 8-Strip tubes,Non-sterile,960 tubes	3420
SSI-1760-S0	1.2ml 8-Strip tubes,Presterile,960 tubes	3870
SSI-1770-00	1.2ml 12-Strip tubes,Non-sterile,960 tubes	3420
SSI-1770-S0	1.2ml 12-Strip Tubes,Presterile,960 tubes	3870
96孔不透氣封口膜
AC1201-01	Sealing film(50)--不透氣	400
AC1201-02	Sealing film(100)	720
96孔透氣封口膜
AC1200-01	AeraSeal Film(50)--透氣	400
AC1200-02	AeraSeal Film(100)	720
一次性多道移液槍分裝容器
AC1331-01	Multi-Channel Diposable reservoir(10pk)	370
AC1332-02	12-channel reservoir(5/pk.4pk/case)	850
96孔過濾板
FL9601	E-Z 96 Lysate Clearance Plate(10)	1800
FL9602	E-Z 97 Lysate Clearance Plate(100)	12000
勻漿柱
HCR003	Homogenizer Spin Column(200)	1000
HCR9601-02	EZ 96 Homogenization plate(4)	550
HCR9601-01	EZ 96 Homogenization plate(1)	150
Dnase試劑盒：用于RNA提取時(shí)DNA的膜上消化以去除DNA污染
E1091-01	Rnase Free Dnase Set(50)	500
E1091-02	Rnase Free Dnase Set(200)	1800
Rnase A:(25mg/ml)在DNA制備時(shí)酶切去除RNA
AC117	RNase A(400ul)	150
AC118	RNase A(5ml)	1050
Proteinase K:在DNA或RNA制備時(shí)酶消化樣品
AC110	Proteinase K,30mg(配buffer1.5ml)	300
AC111	Proteinase K,100mg(配buffer5ml)	780
AC112	Proteinase K,1g(配buffer50ml)	5000
OB Protease:在DNA或RNA制備時(shí)，消化樣品
AC113	OB Protease,30mg(配buffer1.5ml)	300
AC114	OB Protease,100mg(配buffer5ml)	780
溶菌酶Lysozyme:酶切消化去除細(xì)菌胞壁
AC141	Lysozyme A(50mg\23500u/mg)	150
AC142	Lysozyme A(50mg X 4)	560
破壁酶Lyticase:酶切消化去除酵母細(xì)胞壁
AC143	LyticaseA(2500u)	150
AC144	LyticaseA(10000u)	560
ATP-Dependent Dnase:可有效的去除質(zhì)粒溶液中的基因組污染
AC160	ATP-Dependent DNase(1000U)	1500
AC161	ATP-Dependent DNase(5000U)	2900
AC162	ATP-Dependent DNase(10000U)	4800
酸洗玻璃珠：用于物理方法裂解培養(yǎng)細(xì)胞，細(xì)菌或真菌細(xì)胞
AC150	0.1mm-0.2mm Glass Beads(50g)	150
AC151	0.1mm-0.2mm Glass Beads(500g)	1050
AC152	0.4mm-0.6mm Glass Beads(50g)	150
AC153	0.4mm-0.6mm Glass Beads(500g)	1050
Oligo(dT)纖維素：用于純化mRNA
R6512-01	Oligo(dt)cellouse(250mg)	1480
R6512-02	Oligo(dt)cellouse(750mg)	4260
質(zhì)粒相關(guān)溶液
PS001	Solution I, 250ml	180
PS002	Solution II, 250ml	180
PS003	Solution III, 250ml	270
PS004	Neutralization Buffer, 250ml	180
PS005	Buffer SE, 250ml	180
PS006	Buffer T1, 250ml	180
PS007	Buffer T2, 250ml	180
PS008	Buffer T3, 250ml	180
PS009	Buffer HB, 250ml	315
PS010	BAC Binding Buffer,250ml	270
PS011	Buffer N3,250ml	270
PS012	ETR Reagent, 100ml	300
PS013	MGC Binding Buffer Concentrate, 20ml	270
PS014	SPM Wash Buffer Concentrate, 40 ml	270
PS015	Buffer GBT, 100 ml	270
PS016	Endotoxin-Free Elution Buffer-100ml	270
RNA相關(guān)溶液
PR021	TRK Lysis Buffer , 100ml	900
PR022	QVL Lysis Buffer , 100ml	900
PR024	Buffer YRL, 100 ml	360
PR025	Buffer BRL, 100 ml	360
PR026	Buffer RB, 100ml	900
PR027	Buffer RPL, 100ml	900
PR028	Buffer RFL, 100ml	900
PR029	Buffer SP, 60ml	720
PR030	RNA Wash Buffer I, 100ml	360
PR031	RNA Wash Buffer II, 100ml	360
PR032	DEPC-treated Water, 100ml	120
PR033	Linear Acrylamide, 10ml	100
PR034	GTC Buffer,100ml	900
PR035	FTL Buffer,100ml	1080
PR036	AP Buffer,55ml	400
DNA、RNA回收純化相關(guān)溶液
PDR040	Binding Buffer, 200ml(XP2)	450
PDR041	Binding Buffer, 500ml	1080
PDR042	Buffer CP, 200ml	450
PDR043	Buffer CP, 500ml	1080
PDR044	DNA Wash Buffer Concentrate, 40ml	108
PDR045	SPW Wash Buffer Concentrate, 25ml	108
PDR046	RWB Wash Buffer Concentrate, 20ml	360
PDR047	Buffer DP, 100ml	270
PDR048	Elution Buffer, 100ml	150
PDR049	Poly-Gel DNA Elution Buffer, 100ml	180
PDR050	Buffer RFE, 100ml	270
基因組DNA相關(guān)溶液
PD061	Buffer TL,100ml(WTL)	540
PD062	Buffer BL,100ml	900
PD063	Buffer STL,100ml	540
PD064	Buffer BDL,100ml	900
PD065	Buffer BTL,200ml	540
PD066	Buffer YL,100ml	540
PD067	Buffer MTL,100ml	540
PD068	Buffer YDL,100ml	450
PD069	Buffer ERL,100ml-紅細(xì)胞裂解液	540
PD070	Buffer MSL,100ml	270
PD071	Buffer NL,100ml	270
PD072	Buffer XL,100ml	405
PD073	Buffer SP1,250ml	495
PD074	Buffer SP2, 60ml	405
PD075	Buffer SP3, 100ml	405
PD076	Buffer P1, 250ml	495
PD077	Buffer P2, 60ml	405
PD078	Buffer P3, 100ml	405
PD079	Buffer CPL, 100ml	405
PD080	Buffer CXD, 100ml	405
PD081	Buffer SFG1, 250ml	405
PD082	Buffer SFG2, 60ml	405
PD083	Buffer SFG3, 100ml	405
PD084	Buffer FG1, 250ml	405
PD085	Buffer FG2, 60ml	455
PD086	Buffer FG3, 100ml	510
PD087	Buffer GPS 15ml	60
PD088	Buffer GPS 30ml	100
PD089	HTR 50ml	1800
PD090	Buffer SLX mlus,250ml	540
PD091	Buffer SLX ,100ml	900
PD092	Buffer DS 100ml	540
PD093	Midi Syringe	27
PD094	Max Syringe	45
PD095	ETR Binding Buffer 1L	2500
PD096	ETR Wash Buffer 1L	2100
PD097	EHB Buffer 1L	1260
SSI-1014-39	Homogenization Pestles(10/bag,20bag/cs)-0.5ml	1240
SSI-1015-39	Homogenization Pestles(10/bag,20bag/cs)-1.5ml	1240
PD098	玻璃奶 1ml	250
PD099	糖元 10mg	300
PD100	Carrier RNA 1mg	150
PD101	99.8%鹽酸胍(500g)	350
PD102	99.5%異硫氰酸胍(500g)	650
代理價(jià)	單獨(dú)購(gòu)買柱子價(jià)格：盒子的市場(chǎng)價(jià)X0.7X0.55/次數(shù)	質(zhì)粒、PCR、膠回收是2元/個(gè)
市場(chǎng)價(jià)	單獨(dú)購(gòu)買柱子價(jià)格：盒子的市場(chǎng)價(jià)X0.7/次數(shù)	質(zhì)粒、PCR、膠回收是2.5元/個(gè)